Agarose‐based hydrogels with tunable, charge‐selective permeability properties

Physiologically, a hallmark of biological hydrogels is their ability to selectively trap diffusing molecules and particles. And indeed, there now increasing interest in using selective hydrogel barriers for applications biomedicine medical engineering. However, when employing synthetic polymers create with permeabilities, controlling the type strength ensuing filtration process difficult. Here, we generate hybrid gels adjustable selectivity profiles by mixing series (bio-)macromolecules agarose. Depending on concentration incorporated macromolecules, those achieve retardation diffusive translocation either positively or negatively charged dextrans at both, acidic neutral pH. Furthermore, demonstrate three strategies that provide sequestered patches cationic anionic binding sites, thus creating symmetric charge (i.e., electrostatic bandpass) filters which still allow pass. Moreover, such agarose matrices offer high level versatility as permeability can be tailored will integrating macromolecules desired physico-chemical properties. Thus, agarose-based may serve powerful platform engineer versatile materials broad range future field biomedical